Invitrogen™ No-Stain™ Protein Labeling Reagent

Description

• Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer
• Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer
• Easy to use protocol—Mix and incubate with the transferred membrane
• The reaction time is 10 minutes
• Gel staining is also a 10 minute reaction
• Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image
• Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well
• Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection
• Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.