PI15165
Thermo Scientific™ HisProbe™-HRP Conjugate
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 1 | PI15165-Pack-of-1 | In Stock | ₹ 29,603.76 |
PI15165 - Pack of 1
In Stock
Quantity
1
Base Price: ₹ 29,603.76
GST (18%): ₹ 5,328.677
Total Price: ₹ 34,932.437
Description
Pierce HisProbe-HRP Conjugate
Detection Method
Chemiluminescence
Product Line
HisProbe™
Reactivity
All species
Product Type
HRP Conjugate
Form
Solid
Quantity
2 mg
Content And Storage
Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.
Related Products
Description
- Thermo Scientific™ HisProbe-HRP is an nickel (Ni 2 +)-activated derivative of horseradish peroxidase (HRP)
- This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins
- Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags
- The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate
- The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules
- The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC)
- Highlights: Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies
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Description: Pierce HisProbe-HRP Conjugate
Detection Method: Chemiluminescence
Product Line: HisProbe™
Reactivity: All species
Product Type: HRP Conjugate
Form: Solid
Quantity: 2 mg
Content And Storage: Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.
Description:
Pierce HisProbe-HRP Conjugate
Detection Method:
Chemiluminescence
Product Line:
HisProbe™
Reactivity:
All species
Product Type:
HRP Conjugate
Form:
Solid
Quantity:
2 mg
Content And Storage:
Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.
Description: Pierce SuperSignal West HisProbe Kit
Detection Method: Chemiluminescence
Product Line: HisProbe™, SuperSignal™
Reactivity: All species
Product Type: HisProbe Detection Kit
Form: __
Quantity: 685 mL
Content And Storage: Sufficient For: 62 mini blots or 5000 cm2membrane• HisProbe-HRP Conjugate, 2 mg• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL• SuperSignal West Pico Stable Peroxide Solution, 250 mL• Blocker BSA in TBS (10X), 125 mL• TBS Packs (each makes 500 mL), 10 packs• Surfact-Amps 20 Detergent Solution, 6 x 10 mLStore HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.
Description:
Pierce SuperSignal West HisProbe Kit
Detection Method:
Chemiluminescence
Product Line:
HisProbe™, SuperSignal™
Reactivity:
All species
Product Type:
HisProbe Detection Kit
Form:
__
Quantity:
685 mL
Content And Storage:
Sufficient For: 62 mini blots or 5000 cm2membrane• HisProbe-HRP Conjugate, 2 mg• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL• SuperSignal West Pico Stable Peroxide Solution, 250 mL• Blocker BSA in TBS (10X), 125 mL• TBS Packs (each makes 500 mL), 10 packs• Surfact-Amps 20 Detergent Solution, 6 x 10 mLStore HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.
Description: Clear, 96-well
Detection Method: Colorimetric
Product Line: Pierce
Reactivity: __
Product Type: Microplate
Form: __
Quantity: __
Content And Storage: Note: Plates are activated to 1 μL and supplied pre-blocked with SuperBlock™ Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Description:
Clear, 96-well
Detection Method:
Colorimetric
Product Line:
Pierce
Reactivity:
__
Product Type:
Microplate
Form:
__
Quantity:
__
Content And Storage:
Note: Plates are activated to 1 μL and supplied pre-blocked with SuperBlock™ Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Description: White, 96-well
Detection Method: Chemiluminescence
Product Line: NeutrAvidin Pierce
Reactivity: __
Product Type: Microplate
Form: __
Quantity: __
Content And Storage: Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at ambient temperature.
Description:
White, 96-well
Detection Method:
Chemiluminescence
Product Line:
NeutrAvidin Pierce
Reactivity:
__
Product Type:
Microplate
Form:
__
Quantity:
__
Content And Storage:
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at ambient temperature.