Invitrogen™ Bandmate™ Automated Western Blot Processor

Description

• The Bandmate Automated Western Blot Processor is a benchtop instrument that automatically performs the hands-on steps of western blot processing, including blocking, washing, and antibody incubations
• The Bandmate processor requires no special reagents and is programmable, so minimal effort is required for setup and to process your blots
• Bandmate Automated Western Blot Processor features include: • Completely automated antibody incubation and wash steps • Use as little as 5 mL of antibody for mini blots or 7.5 mL for midi blots • Primary and secondary antibodies are recoverable • Process 1–4 mini or 1–2 midi blots simultaneously • Adjustable, easy-to-modify parameters and protocol steps --Dispensing volumes from 10 to 150 mL with up to 10 repetitions per step --Rocking speeds from 5 to 40 cycles per minute --Incubation/rocking times from 1 min to 96 hours --Programmable rinse step option • Ready to use with no specialized reagents required • Easy to remove processing and collection trays for easy clean-up • Additional trays or replacement accessories can be ordered separately How it works Antibody solutions are prepared and loaded into Bandmate Reagent Tubes, then a pre-programmed or stored custom protocol is selected from the touchpad interface
• Prepared wash buffer is held in a separate bottle
• After the program is started, all processing steps are performed in a synchronized manner without further input needed—just set up and walk away
• As the protocol proceeds, the bottoms of the Bandmate Reagent Tubes are punctured to deliver antibody to the processing trays
• After incubation, antibody is funneled into antibody recovery tubes for reuse in future experiments, if desired
• With this unique dispensing system, antibody does not pass through fixed tubing, minimizing potential cross-contamination
• Consistent result from low antibody volumes The Bandmate processor’s precise timing of incubation steps and uniform reagent volume dispensing helps yield consistent results from blot-to-blot and person-to-person
• The Bandmate processor provides a consistent rocking motion during incubation and wash steps
• The rocking motion combined with the unique curved incubation trays help minimize the required antibody volumes
• Antibody volumes as low as 3.5 mL for mini blots and 5 mL for midi blots can be used
• In addition, the Bandmate processor can be set up to run at room temperature or in a cold room overnight
• For convenience, the final wash buffer is held in the processing tray and the rocking motion is maintained so blots remain saturated and ready for the detection step
• The Bandmate processor comes with everything needed to process up to four mini blots or two midi blots at the same time
• The only required consumables are the Bandmate Reagent Tubes, which are changed with each processing experiment.