Invitrogen™ iBlot™ 2 Starter Kit

Description

• The iBlot 2 Starter Kit includes a iBlot 2 Gel Transfer Device that performs western blotting transfer simply, efficiently, and reliably, within seven minutes without the need for liquid buffers
• The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time
• The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane
• How the iBlot 2 System works Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion unlike conventional protein transfer techniques (including wet, semi-wet, and semi-dry) which use inert electrodes that generate oxygen
• With the iBlot 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer
• Simple, user-friendly system Complete protein transfer in 7 minutes or less High detection sensitivity and even transfers Increased blotting reliability and reproducibility Flexible gel size formats and membrane types Ability to create new custom programs Built-in tutorial and application notes