Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Description

• Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods
• TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA
• Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs
• TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining
• When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used
• Highlights: HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use Complete – does not require other buffers or addition of hydrogen peroxide