Description

• General description: Cyanocobalamin (B12) is a water soluble vitamin, commonly called as cobalamins. Dairy products, meat and eggs are the major sources of this vitamin.[1]
• Application: Vitamin B12 has been used:as a component of Erdschreiber′s medium[2]as a supplement of Dulbecco′s modified Eagle′s medium used for the isolation of ventricular myocytes from neonatal and adult rats[3]orally administered to male quails to study its role in reducing the negative effects of sodium selenite intoxication, in vivo[4]as a supplement in DMEM/ medium 199 (M199) medium for differentiation of human myoblasts.[5]
• Biochem/physiol Actions: Vitamin B12 is metabolized to its active form, methylcobalamin.[4] Hindered vitamin B12 uptake is observed in pernicious anemia.[6] Vitamin B12 is essential for erythroblast development. Pregnancy reduces the levels of vitamin B12.[7] Aging reduces vitamin B12 absorption.[8] It works as a coenzyme in fat, carbohydrate and protein metabolism.[4] It is required for growth, genetic stability and survival of cells in vitro. Functions to support one-carbon metabolism. Present in many classical and serum-free formulations.