Anti-Mouse/Rat Foxp3 Staining Set APC, eBioscience™

Description

• The FJK-16s antibody reacts with mouse, rat, dog, porcine, and bovine Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined
• Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice
• Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells
• Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein
• In the human, this region has been shown to be alternatively spliced at the mRNA level
• Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes
• Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human
• Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain
• Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s
• B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s
• Please see our FAQ regarding the usage of eBioscience Foxp3 reagents
• Not included: Fc Block (cat
• 14-0161) Flow Cytometry Staining Buffer (cat
• 00-4222) Rat IgG2a isotype Control (cat
• 12-4321, 11-4321, or 17-4321)