50-169-71

Anti-Mouse/Rat Foxp3 Staining Set FITC, eBioscience™

Manufacturer: Invitrogen

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Pack Size SKU Availability Price
Each 50-169-71-Each In Stock ₹ 33,509.57

50-169-71 - Each

₹ 33,509.57

In Stock

Quantity

1

Base Price: ₹ 33,509.57

GST (18%): ₹ 6,031.723

Total Price: ₹ 39,541.293

Antigen

Foxp3

Classification

Monoclonal

Concentration

0.5mg/mL

Host Species

Rat

Regulatory Status

RUO

Target Species

Mouse, Rat

Isotype

IgG2a κ

Applications

Flow Cytometry

Clone

FJK-16s

Conjugate

FITC

Quantity

Kit

Primary or Secondary

Primary

Content And Storage

Store at 2-8°C. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.

Product Line

eBioscience™

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Description

  • The FJK-16s antibody reacts with mouse, rat, dog, porcine, and bovine Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined
  • Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice
  • Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells
  • Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein
  • In the human, this region has been shown to be alternatively spliced at the mRNA level
  • Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes
  • Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human
  • Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain
  • Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s
  • B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s
  • Please see our FAQ regarding the usage of eBioscience Foxp3 reagents
  • Not included: Fc Block (cat
  • 14-0161) Flow Cytometry Staining Buffer (cat
  • 00-4222) Rat IgG2a isotype Control (cat
  • 12-4321, 11-4321, or 17-4321)

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Antigen:
Foxp3

Classification:
Monoclonal

Concentration:
0.5mg/mL

Host Species:
Rat

Regulatory Status:
RUO

Target Species:
Mouse, Rat

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
FJK-16s

Conjugate:
FITC

Quantity:
Kit

Primary or Secondary:
Primary

Content And Storage:
Store at 2-8°C. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.

Product Line:
eBioscience™

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Antigen:
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Classification:
__

Concentration:
__

Host Species:
__

Regulatory Status:
__

Target Species:
__

Isotype:
__

Applications:
__

Clone:
__

Conjugate:
__

Quantity:
__

Primary or Secondary:
__

Content And Storage:
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Invitrogen

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Antigen:
Foxp3

Classification:
Monoclonal

Concentration:
__

Host Species:
Rat

Regulatory Status:
RUO

Target Species:
Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Non-human Primate, Rhesus Monkey

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
PCH101

Conjugate:
FITC

Quantity:
Kit

Primary or Secondary:
Primary

Content And Storage:
Store at 2-8°C. Do not freeze. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.

Product Line:
eBioscience™

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Biolegend

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Classification:
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Concentration:
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Regulatory Status:
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Isotype:
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Applications:
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Clone:
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Conjugate:
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Quantity:
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Primary or Secondary:
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Content And Storage:
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Product Line:
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