Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 561, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin
• Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis
• Our internal testing shows that Alexa Fluor 561 non-specifically stains B cells in Swiss Webster and SJL mice
• Non-specific staining has not been observed in BALB/c or C57BL/6 mice
• Other strains have not been tested
• See the Antibody Testing Data for an example of this strain-dependent difference
• Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody
• Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610
• Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
• It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
• Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
• It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.