RNA Polymerase II, Mouse anti-Human, Yeast, Clone: 8WG16, Millipore Sigma™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 05952I25UG-Each-of-1 | In Stock | ₹ 17,216.16 |
05952I25UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,216.16
GST (18%): ₹ 3,098.909
Total Price: ₹ 20,315.069
Antigen
RNA Polymerase II
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene Symbols
POLR2A;POLR2
Immunogen
RNA Polymerase II from wheat germ extract
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype
IgG2a κ
Applications
ChIP Assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Clone
8WG16
Dilution
Western Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
Gene Alias
DNA-directed RNA polymerase II subunit RPB1;EC: 2.7.7.6;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Host Species
Mouse
Molecular Weight of Antigen
∽220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_000928
Target Species
Human, Yeast
Form
Purified
Related Products
Description
- Anti-RNA Polymerase II, CTD, clone 8WG16, Cat
- No
- 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting
- DNA-directed RNA polymerase II subunit RPB1 (UniProt: P24928; also known as EC: 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, is encoded by the POLR2A (also known as POLR2) gene (Gene ID: 5430) in human
- DNA-dependent RNA polymerase II catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
- RPB1 subunit is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that suggested to grab the incoming DNA template
- It has multiple zinc and magnesium ion binding sites and contains a repetitive C-terminal domain (CTD; aa 1593-1960) that consists of tandem repeats of the consensus sequence YSPTSPS and serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing
- It can exist in both hypophosphorylated form, which is mainly cytosolic, and in hyperphosphorylated form
- The phosphorylation of Serine 2 and 5 in the hepatapeptide repeat is achieved by Cdk7 and Cdk9
- Cdk7 phosphorylation promotes transcription initiation by triggering promoter release and elongation.
Compare Similar Items
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Antigen: RNA Polymerase II
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene Symbols: POLR2A;POLR2
Immunogen: RNA Polymerase II from wheat germ extract
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG2a κ
Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Clone: 8WG16
Dilution: Western Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
Gene Alias: DNA-directed RNA polymerase II subunit RPB1;EC: 2.7.7.6;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Host Species: Mouse
Molecular Weight of Antigen: ∽220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_000928
Target Species: Human, Yeast
Form: Purified
Antigen: ERK1/MAPK3
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Rabbit monoclonal antibody IgG hybridoma culture supernatant without preservatives.
Gene Symbols: __
Immunogen: KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat ERK-1 (MAPK 3).
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone UP-158-22-3-10 recognizes a C-terminal epitope of MAPK 3 (ERK1). Immunogen sequence is not present in human MAPK3 spliced isoform 3 (ERK1b).
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: __
Applications: Western Blot
Clone: UP-158-22-3-10
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_059043
Target Species: Human
Form: Culture Supernatant
Antigen: __
Classification: __
Conjugate: __
Formulation: __
Gene Symbols: __
Immunogen: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Dilution: __
Gene Alias: __
Host Species: __
Molecular Weight of Antigen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Form: __
