IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™, Invitrogen™

Description

• Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10)
• Mouse IL-10 is an ∽18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF)
• In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10
• IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response
• Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity
• Applications Tested: This JES5-16E3 antibody has been pre-titrated and tested by intracellular staining for flow cytometric analysis
• test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood cells
• This can be used at test size: 5 μL (0.25 μg)/test per test
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
• This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
• It also enhances B cell survival, proliferation, and antibody production
• Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
• Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
• Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
• Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.