IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), APC, eBioscience™, Invitrogen™

Description

• Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2
• This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S)
• This antibody is suitable for both surface and intracellular staining
• This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901) Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis
• Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2 cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser IL-33R, also known as ST2, is a receptor for IL-33
• It is a member of the IL-1 receptor family of innate receptors
• Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33
• IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways
• This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13
• Two most common isoforms of IL-33R that result from alternative splicing are ST2L – the membrane bound form, and ST2S – the soluble form
• The membrane bound form can be released from the cell surface by proteolytic cleavage
• The soluble forms have been suggested to act as decoys dampening the IL-33 signaling
• IL-33R is primarily expressed by mast cells, basophils, and eosinophils
• In mouse, IL-33R expression has been shown on ILC2 and Th2 cells
• IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.