CD130 (GP130) Monoclonal Antibody (KGP130), PE, eBioscience™, Invitrogen™

Description

• Description: The KGP130 monoclonal antibody reacts with mouse gp130, also known as CD130
• gp130 is a 130 kD Type-I transmembrane glycoprotein containing a 597 amino acid extracellular domain, a single transmembrane domain, and a 277 amino acid cytoplasmic domain
• gp130 is a subunit of several heterodimeric cell-surface receptors, including receptors for IL-6, IL-11, IL-27, Oncostatin M, and Leukemia Inhibitory Factor (LIF)
• The gp130 protein has also been found to exist in a soluble form, which is capable of inhibiting IL-6 signaling
• gp130 is expressed mainly on T cells, monocytes, endothelial cells, activated B cells, and plasma cells, and is expressed at lower levels on most leukocytes and epithelial cells
• In response to ligand binding, gp130 becomes tyrosine phosphorylated, leading to activation of several signaling pathways including the PI3 kinase, Ras-MAPk and Stat pathways
• Preincubation of mouse splenic T cells with IL-6 is capable of blocking the interaction of KGP130 with gp130, suggesting that the KGP130 binding site lies near the IL-6 interaction domain
• Reactivity of KGP130 towards human gp130 has not been observed
• Applications Reported: This KGP130 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This KGP130 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM)
• This protein functions as a part of the cytokine receptor complex
• The activation of this protein is dependent upon the binding of cytokines to their receptors
• vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein
• Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis
• Alternatively spliced transcript variants encoding distinct isoforms have been described.