Ubiquitin Monoclonal Antibody (Ubi-1), Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 13-160-0-Each-of-1 | In Stock | ₹ 38,502.00 |
13-160-0 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,502.00
GST (18%): ₹ 6,930.36
Total Price: ₹ 45,432.36
Antigen
Ubiquitin
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.1% sodium azide; pH 7.4
Gene Accession No.
P0CG47, P0CG48, P0CG49, P0CG50, P0CG53, P0CG62, P0CH28, P62979, P62983, P62984, P62985, P62987, P62992, P63048, P79781, Q3T0B7, Q6ZWY3, Q71UM5
Gene Symbols
RPS27A, rps27a.S, RPS27L, UBA52, UBB, UBC
Immunogen
Purified bovine ubiquitin conjugated to carrier protein
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Bovine, Chicken, Human, Mouse, X. laevis
Product Type
Antibody
Isotype
IgG1 κ
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone
Ubi-1
Conjugate
Unconjugated
Gene
UBB
Gene Alias
0610006J14Rik; 1810034D23Rik; 2700054O04Rik; 40S ribosomal protein S27a; 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; AI194771; AL033289; CEP80; CG5271; CG5271-PA; Chap1; Dmel\CG5271; Dmel_CG5271; Dub80; epididymis luminal protein 112; epididymis secretory protein Li 50; HEL112; HEL-S-50; hm:zeh0386; HMG20; hPLIC-2; HRIHFB2157; HUBCEP52; ik:tdsubc_1f2; l(2)04820; l(2)31Ei; LOC100912032; LOC101747587; male female sterile 31; mfs(2)31; mfs(2)48; mfs48; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; poly-ubiquitin precursor; polyubiquitin-B; Polyubiquitin-C; polyubiquitin-C-like; RE44350p; ribosomal protein S27; ribosomal protein S27 like; ribosomal protein S27A; ribosomal protein S27a L homeolog; ribosomal protein S27a S homeolog; ribosomal protein S27-like; ribosomal protein S27-like protein; RPL40; RpS27; Rps27a; rps27a.L; rps27a.S; RpS27A-PA; RPS27L; S27A; similar to polyubiquitin; similar to ubiquitin-S27a fusion protein; Small ribosomal subunit protein eS27-like; Small ribosomal subunit protein eS31; tdsubc_1f2; TI-225; UB3-D; Uba52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP80; Ubi-f80; Ubi-m; Ubiq; Ubiquilin 2; ubiquitin; ubiquitin (34 AA) (1 is 2nd base in codon); ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 80; ubiquitin copy 4; ubiquitin fusion 80; ubiquitin polyprotein (heat shock related); ubiquitin/ribosomal protein; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-CEP80; ubiquitin-hybrid protein precursor; Ubiquitin-monomeric; Ubiquitin-related; Ubiquitin-related 1; Ubiquitin-related 2; ubiquitin-S27a fusion protein; Unknown (protein for MGC:127652); unnamed protein product; XELAEV_18028796mg; xx:tdsubc_1f2; zgc:66168; zgc:66168 protein
Host Species
Mouse
Purification Method
purified
Regulatory Status
RUO
Gene ID (Entrez)
101747587, 22186, 22187, 22190, 281370, 286839, 395796, 395958, 396190, 415372, 444494, 444874, 51065, 614220, 615199, 6233, 67941, 7311, 7314, 7316, 78294
Content And Storage
-20°C
Form
Liquid
Related Products
Description
- 13-1600 recognizes ubiquitin, both conjugated and unconjugated
- It reacts with a single chain 8.5 kDa protein
- The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals
- Ubiquitin is present in the nucleus, cytoplasm, and on cell surface membranes
- This antibody is suitable for immunohistochemical staining of alcohol- or paraformaldehyde-fixed, paraffin-embedded or frozen tissue sections
- Heat-induced epitope retrieval with citrate buffer, pH 6.0, is required for specific staining of formalin-fixed, paraffin-embedded tissue sections
- To stain, incubate 30-60 minutes at room temperature or overnight at 4°C
- This antibody may also be used in ELISA and has been successfully used in western blot analysis of ubiquitinated proteins in mouse thymocytes
- To perform western blotting, prepare lysis buffer in 10 mM N-Ethylmaleimide to inhibit ubiquitin-conjugating enzymes
- N-Ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls
- After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6 M guanidine-HCl, 20 mM Tris-HCl, pH 7.5, 5 mM betamercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4°C, followed by extensive PBS washing
- Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein
- This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus
- When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a
- Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins
- It contains C4-type zinc finger domains and is located in the cytoplasm
- Pseudogenes derived from this gene are present in the genome
- As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi
- Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
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Antigen: Ubiquitin
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.1% sodium azide; pH 7.4
Gene Accession No.: P0CG47, P0CG48, P0CG49, P0CG50, P0CG53, P0CG62, P0CH28, P62979, P62983, P62984, P62985, P62987, P62992, P63048, P79781, Q3T0B7, Q6ZWY3, Q71UM5
Gene Symbols: RPS27A, rps27a.S, RPS27L, UBA52, UBB, UBC
Immunogen: Purified bovine ubiquitin conjugated to carrier protein
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Bovine, Chicken, Human, Mouse, X. laevis
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: Ubi-1
Conjugate: Unconjugated
Gene: UBB
Gene Alias: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; 40S ribosomal protein S27a; 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; AI194771; AL033289; CEP80; CG5271; CG5271-PA; Chap1; Dmel\CG5271; Dmel_CG5271; Dub80; epididymis luminal protein 112; epididymis secretory protein Li 50; HEL112; HEL-S-50; hm:zeh0386; HMG20; hPLIC-2; HRIHFB2157; HUBCEP52; ik:tdsubc_1f2; l(2)04820; l(2)31Ei; LOC100912032; LOC101747587; male female sterile 31; mfs(2)31; mfs(2)48; mfs48; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; poly-ubiquitin precursor; polyubiquitin-B; Polyubiquitin-C; polyubiquitin-C-like; RE44350p; ribosomal protein S27; ribosomal protein S27 like; ribosomal protein S27A; ribosomal protein S27a L homeolog; ribosomal protein S27a S homeolog; ribosomal protein S27-like; ribosomal protein S27-like protein; RPL40; RpS27; Rps27a; rps27a.L; rps27a.S; RpS27A-PA; RPS27L; S27A; similar to polyubiquitin; similar to ubiquitin-S27a fusion protein; Small ribosomal subunit protein eS27-like; Small ribosomal subunit protein eS31; tdsubc_1f2; TI-225; UB3-D; Uba52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP80; Ubi-f80; Ubi-m; Ubiq; Ubiquilin 2; ubiquitin; ubiquitin (34 AA) (1 is 2nd base in codon); ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 80; ubiquitin copy 4; ubiquitin fusion 80; ubiquitin polyprotein (heat shock related); ubiquitin/ribosomal protein; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-CEP80; ubiquitin-hybrid protein precursor; Ubiquitin-monomeric; Ubiquitin-related; Ubiquitin-related 1; Ubiquitin-related 2; ubiquitin-S27a fusion protein; Unknown (protein for MGC:127652); unnamed protein product; XELAEV_18028796mg; xx:tdsubc_1f2; zgc:66168; zgc:66168 protein
Host Species: Mouse
Purification Method: purified
Regulatory Status: RUO
Gene ID (Entrez): 101747587, 22186, 22187, 22190, 281370, 286839, 395796, 395958, 396190, 415372, 444494, 444874, 51065, 614220, 615199, 6233, 67941, 7311, 7314, 7316, 78294
Content And Storage: -20°C
Form: Liquid
Antigen: __
Classification: __
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Content And Storage: __
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Antigen: __
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Immunogen: __
Quantity: 1 mg
Primary or Secondary: __
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Product Type: Tertiapin-Q
Isotype: __
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Clone: __
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Content And Storage: Store at -20°C
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