E-cadherin Monoclonal Antibody (ECCD-1), Invitrogen™

Description

• Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Wistar rat after immunization with mouse teratocarcinoma cell line F9
• Specificity: This antibody specifically reacts with mouse E-cadherin
• This antibody inhibits E-cadherin dependent cell-cell contact
• Cross reactivity: This antibody does not react with human, bovine, chicken or dog E-cadherin
• Storage: 4°C This product does not contain preservative
• The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide
• Avoid repeated freeze-thaw cycles
• Reconstitution: Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL)
• This solution can be used as a stock solution
• If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use
• When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution
• Be sure to store the antibody at a minimum concentration of 2.0 mg/mL
• A lower antibody concentration may result in decreased stability
• Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C
• E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily
• The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer
• Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis
• The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization
• Identified transcript variants arise from mutation at consensus splice sites
• E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity
• In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16
• Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.