E-cadherin Monoclonal Antibody (SHE78-7), Invitrogen™

Description

• Source: Monoclonal antibody was obtained by fusing the P3U1 myeloma cell line with spleen cells of BALB/c mouse after immunization with soluble E-cadherin from human placenta
• Specificity: This antibody specifically reacts with human E-cadherin
• This antibody inhibits E-cadherin dependent cell-cell contact
• Cross reactivity: This antibody does not react with rat E-cadherin
• Storage: 4°C This product does not contain preservative
• The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide
• Avoid repeated freeze-thaw cycles
• Reconstitution: Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL)
• This solution can be used as a stock solution
• If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use
• When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution
• Be sure to store the antibody at a minimum concentration of 2.0 mg/mL
• A lower antibody concentration may result in decreased stability
• Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C
• Dilution solution: 20 mM TBS (pH 7.5) *1 10 mM CaCl2 1.0% BSA (0.1% NaN3) *2 *1 TBS should be used for dilution, as PBS causes precipitation
• Source: Monoclonal antibody was obtained by fusing the P3U1 myeloma cell line with spleen cells of BALB/c mouse after immunization with soluble E-cadherin from human placenta
• Specificity: This antibody specifically reacts with human E-cadherin
• This antibody inhibits E-cadherin dependent cell-cell contact
• Cross reactivity: This antibody does not react with rat E-cadherin
• Storage: 4°C This product does not contain preservative
• The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide
• Avoid repeated freeze-thaw cycles
• Reconstitution: Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL)
• This solution can be used as a stock solution
• If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use
• When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution
• Be sure to store the antibody at a minimum concentration of 2.0 mg/mL
• A lower antibody concentration may result in decreased stability
• Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C
• Dilution solution: 20 mM TBS (pH 7.5) *1 10 mM CaCl2 1.0% BSA (0.1% NaN3) *2 *1 TBS should be used for dilution, as PBS causes precipitation
• *2 When stored at 4C, 0.1% sodium azide should be added as a preservative
• Ca2+ should be contained in the buffers to stabilize cadherin antigen.