CD42d Monoclonal Antibody (1C2), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 1C2 recognizes mouse and rat CD42d, a single transmembrane protein also known as platelet glycoprotein V (GPV)
• CD42d interacts with CD42a, CD42b and CD42c to form the receptor for von willebrand factor and thrombin
• This functional complex plays a role in platelet adhesion
• Expression of CD42d (unlike other markers such as CD41) is restricted to resting platelets and megakaryocytes and therefore is a good marker for development of megakaryocytes
• Applications Reported: This 1C2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections
• Applications Tested: This 1C2 antibody has been tested by flow cytometric analysis of mouse platelets
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells
• NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions
• Monoclonal antibodies have been used to identify different surface antigens present on NK cells
• These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets
• The Cluster of Differentiation (CD) nomenclature was established to standardize the naming of NK cells.