CD95 (APO-1/Fas) Monoclonal Antibody (DX2), eBioscience™, Invitrogen™

Description

• Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily
• CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts
• Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance
• DX2 does not block binding of EOS9.1, another antibody specific for human CD95
• Applications Reported: The DX2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
• DX2 has also been reported in in vitro studies of Fas-induced apoptosis
• EOS9.1 is recommended for induction of apoptosis in vitro
• Applications Tested: The DX2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily
• FAS also exists in a soluble form that weight approximately 26kDa
• Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases
• The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis
• FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells
• At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described
• The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform
• The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer
• It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.