IgM Monoclonal Antibody (HIS40), eBioscience™, Invitrogen™

Description

• Description: The HIS40 monoclonal antibody reacts with the μ heavy chain of rat IgM
• It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells
• Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies
• Applications Reported: The HIS40 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
• Applications Tested: The HIS40 antibody has been tested by flow cytometric analysis of rat splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats
• It plays a crucial role in the immune system by recognizing and neutralizing antigens, such as bacteria and viruses
• Rat IgM has been extensively studied and is widely used in research applications, including immunology, microbiology, and biotechnology
• It is commonly used as a control in experiments, as well as a diagnostic tool for detecting infectious diseases and autoimmune disorders
• Researchers have developed various techniques for producing and purifying Rat IgM, including hybridoma technology, protein A/G chromatography, and affinity chromatography
• Rat IgM is a valuable tool in the field of immunology, and its use has contributed to a better understanding of the immune system and the development of new treatments for various diseases.