Oligodendrocyte Marker O1 Monoclonal Antibody (O1), eBioscience™, Invitrogen™

Description

• Description: This O1 monoclonal antibody reacts with galactocerebroside and other lipids found on late oligodendrocyte precursors
• O1 has been reported to react to all species tested (rat, mouse, human, chicken)
• Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction
• O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors
• O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes
• Applications Reported: This O1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, ELISA, and immunocytochemistry
• Applications Tested: This O1 antibody has been tested by immunocytochemistry on formaldehyde-fixed, differentiated OLN93 cells and can be used at less than or equal to 10 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction
• O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors
• O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.