IgM Monoclonal Antibody (SA-DA4), eBioscience™, Invitrogen™

Description

• The SA-DA4 MAb reacts with the mu heavy chain of human IgM
• It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells
• Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific
• This variation can be attributed to both donor variability and the anticoagulant used during blood draw
• Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody
• Applications Reported: The SA-DA4 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA
• It has also been reported for use in affinity purification of human IgM
• Applications Tested: The SA-DA4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.