IgM Monoclonal Antibody (eB121-15F9), Biotin, eBioscience™, Invitrogen™

Description

• Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM
• It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells
• Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM
• Applications Reported: eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs
• It can also be used in ELISA
• Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen
• It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum
• Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens
• It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated
• Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.