CD207 (Langerin) Monoclonal Antibody (eBioRMUL.2), eBioscience™, Invitrogen™

Description

• RMUL.2 is a MAb that reacts with the predicted extracellular domain of mouse CD207, also known as Langerin
• Mouse CD207 is expressed in a distinct subset of dendritic cells called Langerhans cells (LC)
• Mouse CD207 is a 48 kDa, C-type lectin and putative transmembrane protein that likely plays a role in antigen recognition and uptake
• LC are located in the epidermis and upon activation reduce expression of CD207 and begin migrating through the dermis towards lymphatic vessels
• Expression of mouse CD207 in LC has been correlated to the presence of Birbeck Granules
• Furthermore, the formation of Birbeck Granules has been observed upon transfection of CD207 cDNA into fibroblast cell lines
• Western Blotting with RMUL.2 reveals a 48 kDa band in lysate from mouse ear epidermis as well as in lysates from cells transfected with mouse CD207 cDNA
• Cross reactivity of this antibody with other proteins has not been determined
• Preliminary data from mouse CD207 cDNA transfected cells suggests that RMUL.2 can be used for intracellular staining followed by flow cytometric analysis
• We have observed in our transfectants the majority of protein is expressed intracellularly with a small percentage on the cell surface
• In addition, RMUL.2 has also been tested by immunohistology on mouse ear epidermal sheets and shows specific staining
• Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells
• Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD)
• Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules
• Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG)
• Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.