IL-23 p19 Monoclonal Antibody (5B2), eBioscience™, Invitrogen™

Description

• Description: The 5B2 antibody reacts with the p19 subunit of mouse IL-23
• The 5B2 antibody in combination with the C17.8 antibody yields an improved IL-23 sandwich ELISA with greater sensitivity than was achievable with previous assays
• The 5B2 antibody is a non-neutralizing antibody
• IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19
• p19, like p35 of IL-12, is biologically inactive by itself
• IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R
• IL-23 is secreted by activated mouse and human dendritic cells
• Biological activities of mouse IL-23 are distinct from those of mouse IL-12
• Mouse IL-23 was found not to induce significant amounts of IFN gamma
• Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells
• Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17
• Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN gamma production by naive and memory T cells, as compared to IL-12
• Applications Reported: The anti-mouse p19 5B2 antibody has been reported for use as the capture antibody for a mouse IL-23 sandwich ELISA, when used in conjunction with the anti-p40 C17.8 antibody as the detector
• IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19
• p19, like p35 of IL-12, is biologically inactive by itself
• IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R
• IL-23 is secreted by activated mouse and human dendritic cells
• Biological activities of mouse IL-23 are distinct from those of mouse IL-12
• Mouse IL-23 was found not to induce significant amounts of IFN-γ
• Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells
• Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17
• Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.