IDO Monoclonal Antibody (V1NC3IDO), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody V1NC3IDO recognizes human IDO
• IDO (indoleamine-2,3-dioxygenase, INDO, IDO1) is an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines
• IDO is expressed in a wide variety of tissues and cells, including macrophages, plasmacytoid dendritic cells, and several cell lines
• IDO can be induced in many different cell types by IFN gamma or other inflammatory stimuli
• Expression of IDO in antigen presenting cells and tumors is thought to mediate immune suppression through depletion of this essential amino acid and/or by the creation of tryptophan metabolites that cause apoptosis of T cells and induction of regulatory T cells
• This V1NC3IDO antibody reacts with IDO1 and does not cross react with IDO2
• Applications Reported: This V1NC3IDO antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy
• Applications Tested: This V1NC3IDO antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 0.5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin
• By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines
• IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs)
• In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction
• The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms
• Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites
• Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.