Blood Group Antigen H (O) Type 1 Monoclonal Antibody (17-206), eBioscience™, Invitrogen™

Description

• The MAb 17-206 recognizes the human blood group antigen H (O) type 1, a subtype primarily found in epithelial and secretory cells
• The precursor H antigen is modified by addition of saccharides to carbohydrate chains of proteins and lipids on erythrocytes and epithelial cells
• The type, attachment, location, and sequence of saccharides determines the blood group antigen subtype and is mediated by multiple glycosyl transferases
• Depending on ABO blood type, the H antigen is converted into either the A antigen, B antigen, both the A and B antigen, or left unconverted (O antigen)
• Evidence that the blood group antigen H is not expressed in tumor cells and that the level correlates with disease progression suggests that the blood group antigen H may be involved in cell proliferation, cell adhesion, and angiogenesis, although its exact function remains to be determined
• The 17-206 antibody does not recognize blood group antigen H (O) type 2
• The H antigen is a precursor to the ABO blood group antigens, present in people of all common blood types
• The Bombay phenotype (hh) does not express antigen H on red blood cells, and therefore this type will also lack A or B antigens, similar to the O blood group
• However, unlike O group, the H antigen is absent, hence the individuals produce isoantibodies to antigen H as well as to both A and B antigens
• If they receive blood from someone with O blood group, the anti-H antibodies will bind to the H antigen on the red blood cells of the donor blood and destroy the RBCs by complement-mediated lysis
• Therefore, people with Bombay phenotype can receive blood only from other hh donors, although they can donate as though they were type O
• Some individuals with the blood group A1 may also be able to produce anti-H antibodies due to the complete conversion of all the H antigen to A1 antigen
• Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19
• The H locus contains three exons that span more than 5 kb of genomic DNA, and encodes the fucosyltransferase that produces the H antigen on RBCs
• The H antigen is a carbohydrate sequence with carbohydrates linked mainly to protein (with a minor fraction attached to ceramide moiety).