IRF4 Monoclonal Antibody (3E4), eBioscience™, Invitrogen™

Description

• The MAb 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4)
• IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function
• In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation
• IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1
• In T cells, IRF4 has been implicated in regulatory T (Treg), T helper (Th)2, Th9 and Th17 cell development and function
• This transcription factor is upregulated upon T cell activation and is expressed in mature T cells
• Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression
• IRF4 has also been demonstrated to be involved in macrophage polarization and regulation
• Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers
• The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain
• The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes
• IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems
• A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma
• Alternatively spliced transcript variants have been found.