CD227 (Mucin 1) Monoclonal Antibody (SM3), eBioscience™, Invitrogen™

Description

• This SM3 MAb reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family
• Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated
• Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane
• In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm
• High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition
• In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation
• Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary
• Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer
• Please note this antibody sees a distinct epitope from other Mucin 1 antibodies
• MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages
• MUC1 is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated
• MUC1 is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein
• MUC1 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells, and is expressed abundantly in >90% breast carcinomas and metastases
• Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands
• The MUC1 gene contains seven exons and produces several different alternatively spliced variants
• Overexpression, aberrant intracellular localization, and changes in glycosylation of the MUC1 protein have been associated with carcinomas
• Multiple alternatively spliced transcript variants that encode different isoforms of the MUC1 gene have been reported, but the full-length nature of only some has been determined
• Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.