Gata-3 Monoclonal Antibody (TWAJ), eBioscience™, Invitrogen™

Description

• The MAb TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors
• Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation
• Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney
• During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal
• The Gata-3 is also essential for T cell commitment and survival
• In the thymus, expression is found mainly on the CD4 single positive cells
• During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation
• Alternative splice variants have been reported especially in the MCF7 cell line
• The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein
• Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(cat 00-5523) Crossreactivity in rhesus monkeys has been published
• The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins
• Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes
• GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain
• GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages
• GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers
• Labastie et al
• (1994) cloned the human gene and the 5-prime end of the mouse gene
• The human gene comprises 6 exons distributed over 17 kb of DNA
• Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.