TSLP Monoclonal Antibody (eBio28F12 (28F12)), Functional Grade, eBioscience™, Invitrogen™

Description

• The MAb eBio28F12 reacts with and inhibits the bioactivity of mouse TSLP
• Thymic Stromal-Derived Lymphopoetin (TSLP) was first identified in the tissue culture supernatant of a thymic stromal cell line as a growth factor capable of inducing proliferation and differentiation of pre-B cells
• It was later found to be closely related to another stromal cytokine, IL-7, with which it shares overlapping functions
• Along with its proliferative effects on B cells, TSLP induces dendritic cells to support the differentiation of naive T cells towards the Th2 lineage and may also be involved in the development of CD4^+CD25^+ regulatory T cells
• The receptor for TSLP is heterodimeric and shares one subunit, IL-7Ralpha, with IL-7 and other members of the family, while the TSLPR subunit is unique to TSLP
• TSLP expression can be induced by a variety of inflammatory cytokines and TLR ligands
• Expression is regulated by NF-kappaB and has been detected in epithelial cells, stromal cells, and basophils
• Elevated levels of TSLP are associated with asthma and atopic dermatitis, which are both Th2-mediated inflammatory conditions
• It is believed that chronic overexpression of TSLP may result in increased sensitivity to allergens, resulting in susceptibility to these conditions
• TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain
• It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation
• TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis
• It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis
• It is expressed in heart, liver and prostate.