CD85k (Gp49b) Monoclonal Antibody (H1.1), Functional Grade, eBioscience™, Invitrogen™

Description

• The MAb H1.1 recognizes gp49, a receptor with structural similarities to the PIR (paired Ig-activating receptors) and ILT/KIR(killer inhibitory receptors) family of proteins
• Two closely linked genes on mouse chromosome 10 encode the related proteins gp49A and gp49B
• These two receptors are almost 90% identical in their extracellular regions but differ in their cytoplasmic domains; gp49B contains an ITAM domain while gp49A does not and is extremely short
• Expression of gp49 was originally identified on mast and LAK (IL-2 activated NK) cells
• Expression on LAK cells has been shown to be predominantly gp49B with only a small amount of gp49A
• Additionally gp49 is found on myeloid cells
• Detailed function is not known but gp49B can inhibit cellular activation
• The MAb H1.1 recognizes both gp49A and gp49B
• The H1.1 antibody has been shown to have functional activity: when crosslinked along with anti-NK1.1 it can prevent cytokine release in KY-2 cells
• This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4
• The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
• The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response
• The receptor can also function in antigen capture and presentation
• It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity
• Multiple transcript variants encoding different isoforms have been found for this gene
• [provided by RefSeq, Jul 2008].