GM-CSF Monoclonal Antibody (MP1-22E9), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF)
• The MP1-22E9 antibody is a neutralizing antibody
• Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages
• Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma
• GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells
• GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix
• Non-glycosylated GM-CSF is biologically active
• Applications Reported: The MP1-22E9 antibody has been reported for use in ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis
• Applications Tested: The Functional Grade Purified MP1-22E9 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of GM-CSF bioactivity
• The MP1-22E9 antibody at 1.25 μg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse GM-CSF in an MC/9 cell proliferation assay
• Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section
• The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes
• The active form of the protein exists as a homodimer in the extracellular space
• This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia
• Other genes in the cluster include interleukins 4, 5, and 13
• The gene is involved in promoting tissue inflammation
• Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome
• Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.