CD226 (DNAM-1) Monoclonal Antibody (11A8.7.4), APC, eBioscience™, Invitrogen™

Description

• The monoclonal antibody 11A8.7.4 reacts to DNAM-1 also known as CD226
• DNAM 1 is a 65 kD receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes
• DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells
• DNAM-1 is also expressed by a subset of CD3 positive thymocytes
• The ligands for CD226 are the poliovirus receptor (PVR, CD155) and Poliovirus Related Receptor (PPR2, Nectin-2, CD112)
• CD226 is involved in cytolytic activity by interacting with integrins and activating protein tyrosine kinase Fyn
• The 11A8.7.4 antibody has been shown to increase T and NK cell-mediated cytotoxicity
• Applications Reported: This 11A8.7.4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 11A8.7.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes
• DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells
• DNAM-1 is also expressed by a subset of CD3 positive thymocytes.