CD28 Recombinant Mouse Monoclonal Antibody (CD28.2), Alexa Fluor™ Plus 647, Invitrogen™

Description

• Alexa Fluor Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data
• Alexa Fluor Plus antibodies represent an advancement in fluorescent conjugate technology
• Alexa Fluor Plus antibodies provide brighter signal compared to leading Alexa Fluor antibodies, providing you with better signal-to-noise for your critical experiments
• These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro
• Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment
• This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining
• Applications Tested: This CD28.2 antibody has been tested by flow cytometric analysis of human PBMCs
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser Filtration: 0.2 μm post-manufacturing filtered
• CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein
• CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes
• Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells
• In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death
• In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28
• In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR
• The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells
• Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.