200064869
CD11a (LFA-1alpha) Monoclonal Antibody (HI111), NovaFluor™ Blue 610-70S, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD11a (LFA-1alpha)
Concentration
0.8 μg/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
BSA, PBS with 0.09% sodium azide; pH 7.2
Gene Alias
(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
HI111
Applications
Flow Cytometry
Conjugate
NovaFluor Blue 610-70S
Host Species
Mouse
Target Species
Human
Gene Accession No.
P20701
Gene ID (Entrez)
3683
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
ITGAL
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Description
- CD11a (LFA-1alpha) Monoclonal antibody specifically detects CD11a (LFA-1alpha) in Human samples
- It is validated for Flow Cytometry Description: The HI111 monoclonal antibody reacts with human CD11a, the 180 kDa integrin alphaL, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain
- LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function
- CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1
- CD11a is expressed by all leukocytes
- Applications Reported: The HI111 antibody has been reported for use in flow cytometric analysis
- Applications Tested: The HI111 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.8 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer
- NovaFluor dyes are not compatible with DNA intercalating viability dyes
- Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI
- Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
- This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat
- No
- (<a href='https://www.thermofisher.com/order/catalog/product/C001T06F01'>C001T06F01</a>))
- This buffer contains formulation improvements over CellBlox
- CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used
- Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells
- Add 5 μL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
- For single-color controls, use 5 μL of CellBlox Blocking Buffer per 100 μL of cell sample containing 10^3 to 10^8 cells
- NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts
- <a href = https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/novafluor-dyes.html>Learn more </a> Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser
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Antigen: CD11a (LFA-1alpha)
Concentration: 0.8 μg/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: BSA, PBS with 0.09% sodium azide; pH 7.2
Gene Alias: (p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: HI111
Applications: Flow Cytometry
Conjugate: NovaFluor Blue 610-70S
Host Species: Mouse
Target Species: Human
Gene Accession No.: P20701
Gene ID (Entrez): 3683
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: ITGAL
Antigen:
CD11a (LFA-1alpha)
Concentration:
0.8 μg/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
BSA, PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
HI111
Applications:
Flow Cytometry
Conjugate:
NovaFluor Blue 610-70S
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P20701
Gene ID (Entrez):
3683
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
ITGAL
Antigen: Chromogranin B/CHGB (Neuroendocrine Marker)
Concentration: 200 μg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: CCB peptide; CCB peptide long form; CCB peptide short form; CgB; CHGB; chromogranin B; chromogranin B (secretogranin 1); Chromogranin B, parathyroid secretory protein; chromogranin-B; GAWK peptide; Glucagonoma peptide; PE-11; SCG1; Scg-1; secretogranin B; secretogranin I; Secretogranin-1; secretogranin-I; SgI
Isotype: IgG
Purification Method: Protein A/G
Product Type: Antibody
Clone: CHGB/7756
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P05060
Gene ID (Entrez): 1114
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Chgb
Antigen:
Chromogranin B/CHGB (Neuroendocrine Marker)
Concentration:
200 μg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
CCB peptide; CCB peptide long form; CCB peptide short form; CgB; CHGB; chromogranin B; chromogranin B (secretogranin 1); Chromogranin B, parathyroid secretory protein; chromogranin-B; GAWK peptide; Glucagonoma peptide; PE-11; SCG1; Scg-1; secretogranin B; secretogranin I; Secretogranin-1; secretogranin-I; SgI
Isotype:
IgG
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
CHGB/7756
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P05060
Gene ID (Entrez):
1114
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Chgb
Antigen: HDAC7
Concentration: 200 μg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: 5830434K02Rik; HD7; HD7a; hdac4l; Hdac7; hdac-7; Hdac7a; histone deacetylase 7; Histone deacetylase 7A; mFLJ00062
Isotype: IgG
Purification Method: Protein A/G
Product Type: Antibody
Clone: PCRP-HDAC7-1B6
Applications: Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q8WUI4
Gene ID (Entrez): 51564
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: HDAC7
Antigen:
HDAC7
Concentration:
200 μg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
5830434K02Rik; HD7; HD7a; hdac4l; Hdac7; hdac-7; Hdac7a; histone deacetylase 7; Histone deacetylase 7A; mFLJ00062
Isotype:
IgG
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
PCRP-HDAC7-1B6
Applications:
Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q8WUI4
Gene ID (Entrez):
51564
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
HDAC7
Antigen: CD25
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias: ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: CD25-4E3
Applications: Flow Cytometry
Conjugate: Brilliant Violet 605
Host Species: Mouse
Target Species: Human, Non-human Primate
Gene Accession No.: P01589
Gene ID (Entrez): 3559
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: IL2RA
Antigen:
CD25
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
CD25-4E3
Applications:
Flow Cytometry
Conjugate:
Brilliant Violet 605
Host Species:
Mouse
Target Species:
Human, Non-human Primate
Gene Accession No.:
P01589
Gene ID (Entrez):
3559
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
IL2RA