200064900

CD11a (LFA-1alpha) Monoclonal Antibody (HI111), NovaFluor™ Red 725, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD11a (LFA-1alpha)

Concentration

0.6 μg/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

BSA, PBS with 0.09% sodium azide; pH 7.2

Gene Alias

(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Clone

HI111

Applications

Flow Cytometry

Conjugate

NovaFluor Red 725

Host Species

Mouse

Target Species

Human

Gene Accession No.

P20701

Gene ID (Entrez)

3683

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

ITGAL

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Description

  • CD11a (LFA-1alpha) Monoclonal antibody specifically detects CD11a (LFA-1alpha) in Human samples
  • It is validated for Flow Cytometry Description: The HI111 monoclonal antibody reacts with human CD11a, the 180 kDa integrin alphaL, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain
  • LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function
  • CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1
  • CD11a is expressed by all leukocytes
  • Applications Reported: The HI111 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The HI111 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (0.6 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer
  • NovaFluor dyes are not compatible with DNA intercalating viability dyes
  • Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI
  • Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
  • This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat
  • No
  • (<a href='https://www.thermofisher.com/order/catalog/product/C001T06F01'>C001T06F01</a>))
  • This buffer contains formulation improvements over CellBlox
  • CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used
  • Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells
  • Add 5 μL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
  • For single-color controls, use 5 μL of CellBlox Blocking Buffer per 100 μL of cell sample containing 10^3 to 10^8 cells
  • NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts
  • <a href = https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/novafluor-dyes.html>Learn more </a> Excitation: 636 nm; Emission: 727 nm; Laser: 633-640 nm (Red) Laser

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Mouse Monoclonal Antibody...


Antigen:
CD11a (LFA-1alpha)

Concentration:
0.6 μg/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
BSA, PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
HI111

Applications:
Flow Cytometry

Conjugate:
NovaFluor Red 725

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P20701

Gene ID (Entrez):
3683

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
ITGAL

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Antigen:
IL-17A

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
eBio64DEC17

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 786

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
Q16552

Gene ID (Entrez):
3605

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IL17A

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Rat Monoclonal Antibody...


Antigen:
CD197 (CCR7)

Concentration:
0.6 μg/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
BSA, PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
BLR2; Bukitt's lymphoma receptor 2; CC chemokine receptor 7; C-C chemokine receptor type 7; C-C CKR-7; C-C motif chemokine receptor 7; CC-CKR-7; CCR7; CCR-7; CD197; CDw197; chemokine (C-C motif) receptor 7; chemokine (C-C) receptor 7; Cmkbr7; EBI1; Ebi1h; EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
3D12

Applications:
Flow Cytometry

Conjugate:
NovaFluor Yellow 700

Host Species:
Rat

Target Species:
Human

Gene Accession No.:
P32248

Gene ID (Entrez):
1236

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Ccr7

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Antigen:
CD185 (CXCR5)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
Blr1; Blr-1; Burkitt lymphoma receptor 1; burkitt lymphoma receptor 1 homolog; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD185; CD185 antigen; chemochine (C-X-C motif) receptor 5; chemokine (C-X-C motif) receptor 5; CXC; C-X-C chemokine receptor type 5; CXC motif chemokine; C-X-C motif chemokine receptor 5; CXCR5; CXC-R5; CXCR-5; EGK_06966; Fusin; Gpcr6; GPR9; HUMSTR; LESTR; MDR15; MDR-15; Monocyte-derived receptor 15; neurolymphatic receptor; NLR

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
SPRCL5

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 615

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q04683

Gene ID (Entrez):
12145

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CXCR5