25-080-942

CD80 (B7-1) Monoclonal Antibody (2D10.4), PE-Cyanine7, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 25-080-942-Each-of-1 In Stock ₹ 28,748.16

25-080-942 - Each of 1

₹ 28,748.16

In Stock

Quantity

1

Base Price: ₹ 28,748.16

GST (18%): ₹ 5,174.669

Total Price: ₹ 33,922.829

Antigen

CD80 (B7-1)

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2

Gene Accession No.

P33681

Gene Symbols

CD80

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

941

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

2D10.4

Conjugate

PE-Cyanine7

Gene

CD80

Gene Alias

Activation B7-1 antigen, B7, B7 protein, B7.1, B71, B7-1, B7-1 protein, B7-1 protein precursor, BB1, B-lymphocyte activation antigen B7, Cd28l, CD28LG, CD28LG1, CD80, Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1), CD80 anitgen, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), Cd80 molecule, CD80 protein precursor, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, LAB7, Ly53, Ly-53, MIC17, sCD 80, sCD80, secreted B7-1 protein precursor, soluble CD 80, soluble CD80, T-cell costimulatory molecule, T-cell co-stimulatory protein B7-1, T-lymphocyte activation antigen CD80, TS/A-1, TSA1

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily
  • CD80 is expressed by activated B cells, macrophages and dendritic cells
  • In addition, activated T cells express this antigen
  • CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
  • The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
  • Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 2D10.4 antibody has been pre-diluted and tested by flow cytometric analysis of Daudi cell line
  • This may be used at 5 μL (1.0 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (Product No
  • 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
  • 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
  • Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
  • CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
  • The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
  • Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Compare Similar Items

Show Difference

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Invitrogen

25-080-942

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Antigen:
CD80 (B7-1)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2

Gene Accession No.:
P33681

Gene Symbols:
CD80

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
941

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
2D10.4

Conjugate:
PE-Cyanine7

Gene:
CD80

Gene Alias:
Activation B7-1 antigen, B7, B7 protein, B7.1, B71, B7-1, B7-1 protein, B7-1 protein precursor, BB1, B-lymphocyte activation antigen B7, Cd28l, CD28LG, CD28LG1, CD80, Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1), CD80 anitgen, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), Cd80 molecule, CD80 protein precursor, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, LAB7, Ly53, Ly-53, MIC17, sCD 80, sCD80, secreted B7-1 protein precursor, soluble CD 80, soluble CD80, T-cell costimulatory molecule, T-cell co-stimulatory protein B7-1, T-lymphocyte activation antigen CD80, TS/A-1, TSA1

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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Invitrogen

25-086-941

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Antigen:
CD86 (B7-2)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P42081

Gene Symbols:
CD86

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
942

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
IT2.2

Conjugate:
PE-Cyanine7

Gene:
CD86

Gene Alias:
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2

Host Species:
Mouse

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG2b κ

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Invitrogen

25-090-382

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Antigen:
CD90.2 (Thy-1.2)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide, pH 7.2

Gene Accession No.:
P01831

Gene Symbols:
Thy1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
21838

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
30-H12

Conjugate:
PE-Cyanine7

Gene:
Thy1

Gene Alias:
CD90, FLJ33325, T25, Thy 1.2, Thy1, Thy-1, Thy-1 antigen, thy-1 membrane glycoprotein, Thy1.1, Thy1.2, Thy-1.2, thymus cell antigen 1, theta

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2b κ

Img

Invitrogen

25-090-941

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Antigen:
CD90 (Thy-1)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P04216

Gene Symbols:
Thy1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
7070

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
eBio5E10 (5E10)

Conjugate:
PE-Cyanine7

Gene:
Thy1

Gene Alias:
CD7; CD90; CDw90; FLJ33325; membrane glycoprotein; T25; Thy 1.2; THY1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; thy-1 membrane glycoprotein; Thy-1 protein; Thy-1 T-cell antigen; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen

Host Species:
Mouse

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ