CD197 (CCR7) Monoclonal Antibody (3D12), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197
• CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21
• CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane
• CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells
• CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue
• Expression of CCR7 is also found on DC's
• During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation
• Expression of CCR7 on the cell surface can be down regulated upon ligand binding
• Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human perioheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• CD197 (CCR7) Monoclonal antibody specifically detects CD197 (CCR7) in Human samples
• It is validated for Flow Cytometry.