Galectin 9 Monoclonal Antibody (RG9-35), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: This RG9-35 monoclonal antibody recognizes mouse Galectin 9, a member of the S-type lectin family
• Galectin 9 is widely expressed within a majority of hematopoietic cells with the particularly high expression on CD45R(B220)-intermediate pre-B cells
• Applications Reported: This RG9-35 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RG9-35 antibody has been tested by flow cytometric analysis of mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This may be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions
• The protein encoded by this gene is an S-type lectin
• It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency
• Multiple alternatively spliced transcript variants have been found for this gene.