Kappa Light Chain Monoclonal Antibody (TB28-2), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody TB28-2 recognizes the kappa light chain of human immunoglobulin
• Approximately 22-kDa, the Ig kappa chain is comprised of one variable and one constant region
• Two kappa chains together with the heavy chain comprise the whole Ig structure
• Expression of Ig kappa is typically found on more than half of B lymphocytes
• Applications Reported: This TB28-2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This TB28-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa)
• Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region
• The ratio of kappa to lambda is 70:30
• Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas
• Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.