ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488, Invitrogen™

Description

• This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms
• The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed
• Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line)
• Based on sequence homology, reactivity with other species is likely but has not been confirmed
• For Alexa Fluor 488 excitation 495 nm/emission 519 nm
• Each lot of this antibody is tested for specificity by immunofluorescence
• Expires one year from date of receipt when stored as instructed
• ZO-1 forms complexes with either ZO-2 or ZO-3
• In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction
• ZO-1 expression is significantly reduced in many breast cancer lines
• ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue
• ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions
• ZO-1 may be involved in signal transduction at cell-cell junctions
• Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene
• Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.