CD14 Monoclonal Antibody (61D3), Brilliant Ultra Violet™ 661, eBioscience™, Invitrogen™

Description

• The 61D3 mAb reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein
• CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells
• Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14
• Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 61D3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5μL (0.5μg) per test Brilliant Ultra Violet 661 (BUV661) is a tandem dye that emits at 660 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet Laser
• CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
• CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
• CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
• The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
• Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
• Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
• Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.