RONα Mouse, Unlabeled, Clone: 29, BD

Description

• RON (Recepteur d′ Origine Nantaise) is a tyrosine kinase receptor for MSP (Macrophage Stimulating Protein) that belongs to the hepatocyte growth factor receptor family
• RON is synthesized as a single chain glycosylated polypeptide of 1400 amino acids that is cleaved upon maturation into a 150kDa β-subunit with the kinase activity and a 40kDa α-chain
• Both chains are linked by disulfide bonds and the proper arrangement of the α and β heterodimer is necessary for biological activity of RON
• MSP induces the tyrosine kinase activity of RON, triggering a cascade of intracellular signaling that leads to DNA synthesis
• However, RON activation in mouse erythroleukemia cells results in apoptosis
• The replacement of autophosphorylation sites Y1330 and Y1337 abolishes both the mitogenic and apoptotic effects of MSP
• Therefore, autophosphorylation at Y1330 and Y1337 is required for RON's biological activity
• PLCγ, Shc, Grb2, p61, and p65 associate with RON
• Although widely expressed, RON is particularly abundant in epithelial tissues, lung, brain, adrenal glands, gastrointestinal tract, kidney and testis
• Immunofluorescence, Immunoprecipitation, Western Blotting