Stat3 Mouse, Unlabeled, Clone: 84, BD

Description

• The Stat proteins function as both cytoplasmic signal transducers and activators of transcription
• Stat91/84 (the two proteins are the result of alternate splicing-Stat91has an additional 38 C-terminal amino acids) and Stat113 were the first identified members of this protein family
• With the discovery of additional members of the Stat family (Stats3 & 4), the nomenclature has been revised to indicate the Stat family members in the order of their discovery
• Stat 91, 84, and 113 have become Stat1α, Stat1β, and Stat2, respectively
• Stat3 is a 92kDa protein that is activated as a DNA binding protein through tyrosine phosphorylation in response to treatment of cells with EGF and IL-6 but not with IFN-γ
• Stat3 is widely expressed and can bind to DNA in the absence of Stat1α or Stat2
• Stat3 can bind to the sis-inducible element (SIE) site from the c-fos promoter
• The site is similar to the GAS element that is present in IFN-γ induced genes
• It appears that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1
• With the isolation of Stat3 and the discovery of Stat4, it appears that the Stat family may contain many members, each with a characteristic tissue distribution and specific activating ligands
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting