3879029

PKA (RIIα) Mouse, Unlabeled, Clone: 40, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

PKA (RIIα)

Concentration

250μg/mL

Classification

Monoclonal

Host Species

Mouse

Research Discipline

Cell Biology

Formulation

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype

IgG1

Purification Method

Affinity Purified

Clone

40

Applications

Western Blot

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Canine, Human, Mouse, Rat

Immunogen

Human PKA[RIIα] aa. 1-404

Primary or Secondary

Primary

Content And Storage

Store undiluted at -20°C.

Description

  • cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic(C) and regulatory (R)
  • Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ.These subunits define type I and II cAMP-dependent protein kinases
  • Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active
  • Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ)
  • Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity
  • Most cells express both type I and type II PKAs
  • Although the Rα isoforms are ubiquitously expressed, the Rβ isoforms are predominant in nervous and adipose tissues
  • Expression of the RIα subunit is modulated during muscle and adipocyte differentiation in vitro
  • Immunofluorescence, Immunoprecipitation, Western Blotting

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Antigen:
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Concentration:
250μg/mL

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Monoclonal

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Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype:
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Purification Method:
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Clone:
40

Applications:
Western Blot

Conjugate:
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RUO

Target Species:
Canine, Human, Mouse, Rat

Immunogen:
Human PKA[RIIα] aa. 1-404

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Store undiluted at -20°C.

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