3969316
CD25 Rat anti-Mouse, FITC, Clone: 7D4, BD
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD25
Concentration
0.5mg/mL
Classification
Monoclonal
Host Species
Rat
Research Discipline
Immunology
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype
IgM κ
Purification Method
Affinity Purified
Description
IL-2 Receptor α chain, p55
Clone
7D4
Applications
Flow Cytometry
Conjugate
FITC
Regulatory Status
RUO
Target Species
Mouse
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Description
- The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested
- IL-2Rα by itself is not a signaling receptor
- However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2
- Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα
- CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells
- It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus
- Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance
- It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat
- no
- 553068)
- The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat
- no
- 553866), and it blocks binding of IL-2 to CD25
- This antibody is routinely tested by flow cytometric analysis
- Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature
- Host Species: Rat Clone: 7D4 Isotype: IgM, κ Species Reactivity: Mouse Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2 Flow Cytometry
Compare Similar Items
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Antigen: CD25
Concentration: 0.5mg/mL
Classification: Monoclonal
Host Species: Rat
Research Discipline: Immunology
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype: IgM κ
Purification Method: Affinity Purified
Description: IL-2 Receptor α chain, p55
Clone: 7D4
Applications: Flow Cytometry
Conjugate: FITC
Regulatory Status: RUO
Target Species: Mouse
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD25
Concentration:
0.5mg/mL
Classification:
Monoclonal
Host Species:
Rat
Research Discipline:
Immunology
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype:
IgM κ
Purification Method:
Affinity Purified
Description:
IL-2 Receptor α chain, p55
Clone:
7D4
Applications:
Flow Cytometry
Conjugate:
FITC
Regulatory Status:
RUO
Target Species:
Mouse
Immunogen:
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD27
Concentration: __
Classification: Monoclonal
Host Species: Mouse
Research Discipline: Immunology
Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Description: __
Clone: M-T271
Applications: Flow Cytometry
Conjugate: Violet Fluor 450 (V450)
Regulatory Status: RUO
Target Species: Human
Immunogen: Human T-CLL cells
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD27
Concentration:
__
Classification:
Monoclonal
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Description:
__
Clone:
M-T271
Applications:
Flow Cytometry
Conjugate:
Violet Fluor 450 (V450)
Regulatory Status:
RUO
Target Species:
Human
Immunogen:
Human T-CLL cells
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD3
Concentration: __
Classification: Monoclonal
Host Species: Mouse
Research Discipline: Immunology
Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Description: CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Clone: SK7
Applications: Flow Cytometry
Conjugate: APC-H7
Regulatory Status: RUO
Target Species: Human
Immunogen: Human Thymocytes
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD3
Concentration:
__
Classification:
Monoclonal
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Description:
CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Clone:
SK7
Applications:
Flow Cytometry
Conjugate:
APC-H7
Regulatory Status:
RUO
Target Species:
Human
Immunogen:
Human Thymocytes
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD3
Concentration: __
Classification: Monoclonal
Host Species: Mouse
Research Discipline: Immunology
Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Description: __
Clone: UCHT1
Applications: Flow Cytometry
Conjugate: Violet Fluor 450 (V450)
Regulatory Status: RUO
Target Species: Human
Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD3
Concentration:
__
Classification:
Monoclonal
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Description:
__
Clone:
UCHT1
Applications:
Flow Cytometry
Conjugate:
Violet Fluor 450 (V450)
Regulatory Status:
RUO
Target Species:
Human
Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.