3969413

CD3 Mouse anti-Human, V450, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3

Applications

Flow Cytometry

Conjugate

Violet Fluor 450 (V450)

Regulatory Status

RUO

Target Species

Human

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

UCHT1

Classification

Monoclonal

Host Species

Mouse

Research Discipline

Immunology

Formulation

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Isotype

IgG1 κ

Purification Method

Affinity Purified

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Description

  • Reacts with the human ε-chain, a 20kDa subunit of CD3/T cell antigen receptor complex found on 70%-80% of normal human peripheral blood lymphocytes and 60%-85% of thymocytes
  • Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjunction with pokeweed mitogen
  • CD3 plays a role in signal transduction during antigen recognition
  • UCHT1 antibody stains intracellular CD3 unlike the other CD3 clone, HIT3a, which stains only the extracellular CD3
  • The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
  • It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm
  • Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates
  • Host Species: Mouse Clone: UCHT1 Isotype: IgG1 κ Species Reactivity: Human Flow Cytometry, Functional Assay

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Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
Violet Fluor 450 (V450)

Regulatory Status:
RUO

Target Species:
Human

Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
UCHT1

Classification:
Monoclonal

Host Species:
Mouse

Research Discipline:
Immunology

Formulation:
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

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Flow Cytometry

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Rat

Immunogen:
Recombinant Rat CD32 Protein

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C

Clone:
D34-485

Classification:
Monoclonal

Host Species:
Mouse

Research Discipline:
Immunology

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

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Primary or Secondary:
Primary

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Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Classification:
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Purification Method:
Affinity Purified

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Antigen:
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Applications:
Flow Cytometry

Conjugate:
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Regulatory Status:
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Target Species:
Mouse

Immunogen:
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Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
145-2C11

Classification:
Monoclonal

Host Species:
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Research Discipline:
Immunology

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype:
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Purification Method:
Affinity Purified