CD25 Monoclonal Antibody (CD25-4E3), Brilliant Violet™ 605, eBioscience™, Invitrogen™

Description

• CD25 Monoclonal antibody specifically detects CD25 in Human, Non-human Primate samples
• It is validated for Flow Cytometry Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha
• CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages
• This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor
• IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes
• CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes
• This monoclonal antibody is cross-reactive to rhesus monkey
• Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This CD25-4E3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Brilliant Violet™ 605 (BV605) is a tandem dye that emits at 605 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href='https://www.thermofisher.com/order/catalog/product/SB-4401-42'>Product # SB-4401-42</a>) or Brilliant Stain Buffer™ (<a href='https://www.thermofisher.com/order/catalog/product/00-4409-75'>Product # 00-4409-75</a>) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (<a href='https://www.thermofisher.com/order/catalog/product/00-8222-49'>Product # 00-8222-49</a>) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href='https://www.thermofisher.com/order/catalog/product/00-5333-54'>Product # 00-5333-54</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
• Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation
• Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
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