Cytokeratin 18 Monoclonal Antibody (LDK18), eFluor™ 570, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody LDK18 recognizes human Cytokeratin 18
• Cytokeratin 18 is member of the large family of intermediate filaments found within epithelial cells
• The family of cytokeratins is made up of type I (acidic) and type II (basic) members which form heterodimers by pairing an acidic with a basic member to compose the keratin filament
• Cytokeratin 18 (type I) is most commonly found associated with cytokeratin 8 (type II)
• Because many tumors of epithelial origin retain expression of keratin proteins, cytokeratin 18 has been used as a tumor marker to identify both tumors and metastases
• In addition, cytokeratin 18 has been shown to be expressed in highly proliferative retinal pigment epithelium cells
• Applications Reported: This LDK18 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry
• Applications Tested: This LDK18 antibody has been tested by immunocytochemistry of MeOH-fixed human cells and can be used at less than or equal to 1 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70)
• A standard Alexa Fluor™ 555 or TRITC filter is acceptable
• Excitation: 555 nm; Emission: 570 nm Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8
• Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18
• Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas
• Hepatocellular carcinomas express only cytokeratins 8 and 18
• Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis
• Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene
• Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies
• The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.