4325926

CD3e Hamster anti-Mouse, V450, Clone: 500A2, BD

Hamster Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3e

Concentration

0.2mg/mL

Classification

Monoclonal

Host Species

Hamster

Target Species

Mouse

Immunogen

Mouse T-cell receptor

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

500A2

Applications

Flow Cytometry

Conjugate

Violet Fluor 450 (V450)

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Isotype

IgG2 κ

Purification Method

Affinity Purified

Description

CD3ε chain

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Description

  • This antibody has been reported to react with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains
  • Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro
  • Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor
  • This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes
  • The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
  • It is excited by the 450 nm
  • Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

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Fischer Scientific

4325926

Hamster Monoclonal Antibody...


Antigen:
CD3e

Concentration:
0.2mg/mL

Classification:
Monoclonal

Host Species:
Hamster

Target Species:
Mouse

Immunogen:
Mouse T-cell receptor

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
500A2

Applications:
Flow Cytometry

Conjugate:
Violet Fluor 450 (V450)

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Isotype:
IgG2 κ

Purification Method:
Affinity Purified

Description:
CD3ε chain

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Mouse Monoclonal Antibody...


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Concentration:
__

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
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Immunogen:
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Primary or Secondary:
Primary

Content And Storage:
Store undiluted at -20°C.

Clone:
9E10.

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
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Isotype:
IgG1

Purification Method:
Affinity Purified

Description:
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Host Species:
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Content And Storage:
Store undiluted at -20°C.

Clone:
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Applications:
Western Blot

Conjugate:
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RUO

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Antigen:
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Concentration:
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Classification:
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Host Species:
Mouse

Target Species:
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Immunogen:
FoxP3 recombinant protein

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
259D/C7

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 488

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
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Purification Method:
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Description:
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